DNA

Part:BBa_K4342031:Design

Designed by: Samer Salman   Group: iGEM22_Austin_UTexas   (2022-10-12)


nptII Detector Rescue Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3493
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1325
    Illegal XbaI site found at 2537
    Illegal XbaI site found at 3413
    Illegal SpeI site found at 3091
    Illegal PstI site found at 645
    Illegal PstI site found at 1303
    Illegal PstI site found at 1890
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3493
    Illegal NheI site found at 4032
    Illegal SpeI site found at 3091
    Illegal PstI site found at 645
    Illegal PstI site found at 1303
    Illegal PstI site found at 1890
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3493
    Illegal BglII site found at 1677
    Illegal BglII site found at 3251
    Illegal BglII site found at 3749
    Illegal BamHI site found at 1291
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3493
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1325
    Illegal XbaI site found at 2537
    Illegal XbaI site found at 3413
    Illegal SpeI site found at 3091
    Illegal PstI site found at 645
    Illegal PstI site found at 1303
    Illegal PstI site found at 1890
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1040
    Illegal EcoRI site found at 3493
    Illegal XbaI site found at 180
    Illegal XbaI site found at 1325
    Illegal XbaI site found at 2537
    Illegal XbaI site found at 3413
    Illegal SpeI site found at 3091
    Illegal PstI site found at 645
    Illegal PstI site found at 1303
    Illegal PstI site found at 1890
    Illegal NgoMIV site found at 2331
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References